More information about the research carried out by HMohammed Lab.

Mission

To understand the fundamentals of epigenetic and transcriptional regulation in hormone driven cancers such as breast and prostate cancer for early detection and treatment

Primary Areas of Interest

Our research has three primary areas of interest:

1. Cancer epigenetics and steroid hormones

Hormones such as estrogen and androgen have well documented roles in driving cancer. Our lab studies fundamental mechanisms regulating these processes to understand how factors important in normal tissues end up driving cancer. We also develop and apply novel technologies for earlier detection of lethal cancers and to identify therapeutic vulnerabilities as the disease evolves. (Mohammed et al., Nature 2015, Mohammed et al., Cell Reports 2013, Mohammed*, Serandour* et al., Oncogene 2018, Mohammed et al., Molecular Oncology 2018)

2. Proteomics approaches to investigate transcription factor complexes

We developed the RIME as a tool to assay endogenous protein complexes. RIME has been used to study complexes from cancer to embryonic stem cells. We are currently developing nanoparticle based approaches to increase sensitivity of the method and make it applicable to a wider range of biological samples. (Mohammed et al., Nature Protocols 2016, Mohammed et al., Cell Reports 2013, Mohammed et al., Nature 2016)

3. Single-cell multi-omics approaches to connect underlying tumor heterogeneity with hormone response

Using novel single-cell methods, including the multi-omic NMT-seq approach, we are currently profiling tumor cell state at a transcriptional and epigenetic level. In cell line and primary patient samples, we are studying how underlying genetic and epigenetic variations impact function of hormones in cancer.   From a technology development perspective, we are adapting NMT-seq to spatial platforms and also working to improve throughput of the method. (Mohammed*, Argelaguet* et al., Nature 2019, Mohammed*, Irene Herraez*, et al., Cell Reports 2017, Mohammed et al., Cell Systems 2018)

Our Technologies

Single-cell nucleosome, methylation and transcription sequencing

scNMT-seq is a single-cell method for parallel transcriptome, chromatin accessibility, DNA methylation and transcriptome profiling.

Rapid immunoprecipitation mass spectrometry of endogenous protein

RIME (Rapid immunoprecipitation mass spectrometry of endogenous protein) is a method that allows the study of protein complexes, in particular chromatin and transcription factor complexes, in a rapid and robust manner by mass spectrometry (MS).