Decoding the epigenetic logic of hormone-driven cancers
We combine single-cell multiomics and proteomics to understand how genetic and epigenetic heterogeneity shapes hormone response in breast and prostate cancer — toward earlier detection and treatment.
Based at OHSU's Knight Cancer Institute in Portland — a small group studying the epigenetics of hormone-driven cancers, with methods built in the lab: RIME, scNMT-seq and TITAN.
To understand the fundamentals of epigenetic and transcriptional regulation in cellular dynamics — for early detection and treatment.
The lab is based at Oregon Health and Science University's Knight Cancer Institute, dedicated to detecting and eliminating lethal cancers early.
Two complementary lines of inquiry
Methods built in the lab
Single-cell nucleosome, methylation and transcription sequencing
Parallel profiling of the transcriptome, chromatin accessibility, and DNA methylation — in the same single cell.
Read the methodRapid immunoprecipitation mass spectrometry of endogenous proteins
Rapid, robust study of protein complexes — in particular chromatin and transcription factor complexes — by mass spectrometry.
Read the methodTopic inference of transcriptionally associated networks
A topic-modeling machine learning approach that identifies transcriptional cell states in single-cell RNA-seq data.
Read the methodFeatured publications
Multi-omics profiling of mouse gastrulation at single-cell resolution
Argelaguet*, Clark*, Mohammed* et al. — co-first author
Progesterone receptor redirects ERα chromatin binding in breast cancer to elicit good clinical outcome
Mohammed et al.
Estrogen regulates divergent transcriptional and epigenetic cell states in breast cancer
Ors, Chitsazan, Doe, Mulqueen et al. — from the lab
Rapid Immunoprecipitation and Mass Spectrometry of Endogenous Proteins (RIME)
Mohammed et al. — RIME
The people behind the work
Meet the scientists behind the work, and see how to join the lab. We recruit at all levels.