Research
Our mission: to understand the fundamentals of epigenetic and transcriptional regulation in cellular dynamics — for early detection and treatment.
Primary areas of interest
We developed RIME as a tool to assay endogenous protein complexes. RIME has been used to study complexes from cancer to embryonic stem cells. We are currently developing nanoparticle-based approaches to increase the sensitivity of the method and make it applicable to a wider range of biological samples.
Mohammed et al., Nature Protocols 2016 · Mohammed et al., Cell Reports 2013 · Nature 2016
Using novel single-cell methods, including the multiomic NMT-seq approach, we profile tumor cell state at a transcriptional and epigenetic level. In cell lines and primary patient samples, we study how underlying genetic and epigenetic variations impact the function of hormones in cancer. On the technology side, we are adapting NMT-seq to spatial platforms and working to improve its throughput.
Mohammed*, Argelaguet* et al., Nature 2019 · Mohammed* et al., Cell Reports 2017 · Cell Systems 2018
In progress
A look at active projects and the data behind them. Some pieces are interactive — hover and click to explore. Updated as the work develops.
Our technologies
Methods developed by the lab and its members, openly available to the research community.
A single-cell method for parallel transcriptome, chromatin accessibility, and DNA methylation profiling — three layers of regulation from one cell.
Read the methodAllows the study of protein complexes — in particular chromatin and transcription factor complexes — in a rapid and robust manner by mass spectrometry.
Read the methodA topic-modeling-based machine learning approach to identify transcriptional cell states in single-cell RNA-seq datasets.
Read the methodEvery method and finding above is documented in our publication record — explore the full list.